Laser Capture Microdissection: Methods and Protocols (Methods in Molecular Biology)
Graeme I. Murray, Stephanie Curran
Expert laboratorians noted for their optimized methods describe in step-by-step detail how to successfully carry out tissue-based laser microdissection in their own laboratory using the different systems available and to apply a wide range of sophisticated molecular technologies to the microdissected tissue samples. Highlights include gene expression profiling of primary tumor cell populations using laser capture microdissection, RNA transcript amplification and GeneChip® microarrays, laser capture microdissection and colorectal cancer proteomics, and proteomic analysis of human bladder tissue using SELDI® approach following microdissection techniques. Applications of the microdissected tissue described encompass PCR, gene expression studies including microarray analysis, and proteomics.
Catégories:
Année:
2004
Edition:
1
Editeur::
Humana Press
Langue:
english
Pages:
335
ISBN 10:
1592598536
ISBN 13:
9781592598533
Collection:
Methods in Molecular Biology
Fichier:
PDF, 8.80 MB
IPFS:
,
english, 2004